Journal:

Article Title: The Expression and Distribution of the Hypoxia-Inducible Factors HIF-1? and HIF-2? in Normal Human Tissues, Cancers, and Tumor-Associated Macrophages

doi:

Figure Lengend Snippet: Details of the Primary Antibodies Used

Article Snippet: For some of the CD68 immunostaining biotinylated goat anti-mouse serum at 1/400 (DAKO) was used as the secondary followed by streptABComplex/AP (DAKO).

Techniques:

Journal:

Article Title: The Expression and Distribution of the Hypoxia-Inducible Factors HIF-1? and HIF-2? in Normal Human Tissues, Cancers, and Tumor-Associated Macrophages

doi:

Figure Lengend Snippet: Expression of HIF-2α protein in normal bone marrow macrophages and U937 cell line. Normal bone marrow macrophages show immunoreactivity with mAb 190b. In serial paraffin sections of normal bone marrow trephine staining with CD68 (using mAb PGM1; A), and mAb 190b (B) colocalize in macrophages but not megakaryocytes. U937 cells were cultured with or without PMA (1.6 × 10− 8 M) and incubated in normoxia (N) or 0.1% hypoxia (H) for 4 hours. Whole cell extracts (75 μg) were prepared, separated by SDS-PAGE, transferred onto PVDF membrane, and analyzed with mAb 190b (C). For comparison a hypoxic cell extract of HIF-2α transfected HT1080 cells (50 μg) prepared after culture for 4 hours in 0.1% hypoxia was run in parallel (lane C). The dominant band detected in each case comigrated at the mobility predicted for HIF-2α. In both differentiated and undifferentiated U937 cells the band detected showed hypoxic induction. However, after differentiation the normoxic levels seen were comparable with those seen in hypoxia in undifferentiated cells. U937 cells were cultured with PMA (1.6 × 10− 8 M) to allow differentiation into macrophages, incubated in normoxia or placed in 0.1% hypoxia for 4 hours, and immunostained with mAbs to HIF-2α (190b) and CD11c (KB 90). HIF-2α expression was absent after normoxic culture (D) but detectable after hypoxic culture (E). CD11c expression, confirming macrophage differentiation, was unaffected by normoxic/hypoxic culture (not shown). Original magnifications, ×400 (A and B) and ×300 (D and E)

Article Snippet: For some of the CD68 immunostaining biotinylated goat anti-mouse serum at 1/400 (DAKO) was used as the secondary followed by streptABComplex/AP (DAKO).

Techniques: Expressing, Staining, Cell Culture, Incubation, SDS Page, Transfection

Journal: Journal of Virology

Article Title: Brain Large Artery Lymphocytic Inflammation and Human Immunodeficiency Virus-Related Brain Arterial Remodeling

doi: 10.1128/JVI.00081-18

Figure Lengend Snippet: Intima CD3 + /CD68 + cell phenotypes and markers of brain arterial remodeling a

Article Snippet: Briefly, anti-CD68 + antibody (primary, 1:100, catalog number M0814, from Dako Corp., Carpinteria, CA; secondary, biotinylated horse anti-mouse IgG antibody, catalog number BA-2000, from Vector Laboratories, Burlingame, CA) binding to arteries was counterstained with hematoxylin and visualized using the diaminobenzidine kit ( 10 ).

Techniques: Isolation

Journal: Frontiers in Cardiovascular Medicine

Article Title: Small-diameter bacterial cellulose-based vascular grafts for coronary artery bypass grafting in a pig model

doi: 10.3389/fcvm.2022.881557

Figure Lengend Snippet: Inner layer of the vascular grafts 4 weeks after implantation. Representative images of the vascular grafts at the central part (A–C) and at the anastomosis site (D–F) stained with H&E (A,D) and immunofluorescence antibodies specific for Ve-Cadherin (red), α-SMA (cyan), laminin (green) (B,E) , and for CD31 (red) and CD68 (green) (C,F) . Nuclei were stained with DAPI (blue) (B,C,E,F) . Scale bar = 100 μm.

Article Snippet: For immunofluorescence staining, the following antibodies were used: rabbit anti-laminin (ab11575, dilution at 1:200) and goat anti-smooth muscle actin (SMA) (ab7817, dilution at 1:400) from Abcam (UK), goat anti-vascular endothelial (VE) cadherin (sc6458, dilution at 1:100) from Santa Cruz Biotechnology (Dallas, US), mouse anti-CD31 (MCA1746GA, dilution at 1:100) from AbD Serotec (Germany), mouse anti-CD68 (BA4D5, dilution at 1:50) from AbD Serotec (Germany).

Techniques: Staining, Immunofluorescence